Characterization of Embryonic Stem (ES) Neuronal Differentiation Combining Atomic Force, Confocal and DIC Microscopy Imaging
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Differentiation of human embryonic stem cells into neurons
Human embryonic stem (ES) cells are undifferentiated pluripotent cells derived from the inner cell mass of blastocyst stage embryos. These unique cell lines have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. These cells are capable of forming embryoid bodies (EB) that contain cells from all three embryonic lin...
متن کاملDifferentiation of human embryonic stem cells into neurons
Human embryonic stem (ES) cells are undifferentiated pluripotent cells derived from the inner cell mass of blastocyst stage embryos. These unique cell lines have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. These cells are capable of forming embryoid bodies (EB) that contain cells from all three embryonic lin...
متن کاملLive imaging genetically-encoded fluorescent proteins in embryonic stem cells using confocal microscopy
The ability to non-invasively visualize, track and quantify events as they take place in living cells is essential for developing a deeper understanding of biological processes. The recent explosion in the field of stem cell biology, and the therapeutic potential of embryonic and adult stem cells have necessitated the development of a suite of tools for live imaging stem cells. ES cells are der...
متن کاملالقای فنوتیپ عصبی در سلولهای بنیادی رویانی توسط داروی دپرنیل
Introduction & Objective:: Previous studies have shown that ES cells could be induced to differentiate into neurons and gelia in vitro. Induction protocols are based on culture in the presence of an inducer such as RA. In this study, the effect of deprenyl on the differentiation of embryonic stem cells (ES) cells to neuron-like cells was investigated. Deprenyl is a type B monoamine oxidase...
متن کاملThe Effect of Astrocyte-Conditioned Medium (ACM) and Retinoic Acid on Neural Differentiation of Mouse Embryonic Stem Cells
Purpose: The aim of this research was to study the properties of factors secreted from astrocyte cells in suspension medium in direct differentiation of mouse embryonic stem cells into neural cells. Materials and Methods: Royan B1 mouse embryonic stem (ES) cells were used in this experiment. For differentiation of Es cells into the neural cells, the astrocyte-condition medium (ACM) of mouse fe...
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تاریخ انتشار 2017